Study on Chinese Maifanite in Promotion of Heterotrophic Denitrification in Water

doi:10.19657/j.geoscience.1000-8527.2021.105

Abstract

Abstract:

Microbial heterotrophic denitrification technology is widely used in municipal wastewater treatment. However, in practice, microbial activity is readily affected by environmental conditions (e.g., shock load and heavy metal stress), making the effluent unstable. Chinese maifanite, a natural silicate mineral, can effectively improve microbial activity and is resistate to environmental stress. Therefore, it can be used in microbial pollution control. Through batch experiments, the dosage and the effect of treatment methods of natural silicate mineral (Chinese maifanite) on heterotrophic denitrification were studied, and the mechanism of Chinese maifanite on microorganisms was explored. The results show that the dosage and treatment method would significantly affect the nitrate removal by microorganisms. The nitrate removal rate correlates positively with the Chinese maifanite dosage. When the dosage of Chinese maifanite is 350 g/L, the nitrate removal rate can reach 96%, while that of the control group is only 25%. The treatment method would affect the increasing effect of Chinese maifanite on denitrification. The ground Chinese maifanite has an advantage when the dosage is below 150 g/L. With increasing dosage, the untreated Chinese maifanite facilitates denitrification better. The untreated/ground/ball-milled Chinese maifanite extract was furtherly used in the denitrification promotion and microbial toxicity experiments. The experimental results show that the Chinese maifanite extract can improve the microorganism activity and environmental stress resistance. The main biological promotion factor of Chinese maifanite is the composition and concentration of the dissolved trace elements. Our results provide theoretical basis and practical application value for the microbial treatment stimulated by natural Chinese maifanite.

Key words: heterotrophic denitrification, Chinese manifinite, biostimulation, sewage treatment, ball mill

CLC Number:

P641.3
X52

LIU Wenjun, LIU Tong, HU Weiwu, CHEN Nan, FENG Chuanping. Study on Chinese Maifanite in Promotion of Heterotrophic Denitrification in Water[J]. Geoscience, 2022, 36(02): 574-582.

Figures/Tables 11

Fig.1 Effect of CM dosage on N O 3 - (a), N O 2 - (b) and pH (c) in the reaction system

Fig.2 Effect of GCM dosage on N O 3 - (a), N O 2 - (b) and pH (c) in the reaction system

Table 1 Analysis of Anova for the linear model with different CM dosages

投加量/(g/L)	p值	差异性
50	0.365 3	无差异
150	0.042 8	有差异
250	0.000 413	有极其显著差异
350	7.84e-07	有极其显著差异

Table 2 Analysis of Anova for the linear model with different GCM dosages

投加量/(g/L)	p值	差异性
50	0.003 1	有显著差异
150	0.000 15	有极其显著差异
250	8.68e-06	有极其显著差异
350	2.86e-08	有极其显著差异

Fig.3 Linear fitting between the CM/GCM dosage and rate constant

Fig.4 Effect of CM extracts on N O 3 - (a) and N O 2 - (b) in the reaction system

Fig.5 Effect of GCM extracts on N O 3 - (a) and N O 2 - (b) in the reaction system

Fig.6 Effect of BMCM extracts on N O 3 - (a) and N O 2 - (b) in the reaction system

Fig.7 Comparison of kinetic constants

Fig.8 Effects of three extracts with the same TDS on denitrification

Fig.9 Inhibition rate on microorganisms by Chinese maifanite extracts

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