玉米根际土壤细菌群落对不同程度镉污染的响应: 以甘肃省白银市四龙镇地区为例

doi:10.19657/j.geoscience.1000-8527.2023.029

现代地质 ›› 2023, Vol. 37 ›› Issue (03): 758-766.DOI: 10.19657/j.geoscience.1000-8527.2023.029

• 实验、应用与环境地球化学 • 上一篇下一篇

玉米根际土壤细菌群落对不同程度镉污染的响应: 以甘肃省白银市四龙镇地区为例

胡永浩¹(), 段星星²^,³(), 夏昭德¹(), 韩宝华³

1.长安大学地球科学与资源学院,陕西西安 710061
2.中国地质调查局乌鲁木齐自然资源综合调查中心,新疆乌鲁木齐 830000
3.石河子大学,新疆石河子 832003

收稿日期:2022-06-28 修回日期:2023-03-19 出版日期:2023-06-10 发布日期:2023-07-20
通讯作者: 夏昭德,男,副教授,1984年出生,地质学专业,主要从事地球化学和岩浆岩相关教学和研究工作。Email:karlde@163.com。
段星星,男,博士,高级工程师,1983年出生,地质学专业,主要从事地球化学调查和研究。Email:duanxx@foxmail.com。
作者简介:胡永浩,男,硕士研究生,1998年出生,地质学专业,主要从事土壤地球化学。Email:hyh9809@163.com。
基金资助:
新疆维吾尔自治区自然科学基金面上项目“绿洲土壤无机碳碳汇及有效性定量分析”(2022D01A149);新疆乌鲁木齐自然资源中心项目“南疆铁门关地区土地质量生态修复支撑调查”(T14-1-LQ34)

Impacts of Cadmium Concentration in Rhizospheric Soil of Corn on Bacterial Communities: A Case Study from Silong Town, Baiyin City, Gansu Province

HU Yonghao¹(), DUAN Xingxing²^,³(), XIA Zhaode¹(), HAN Baohua³

1. School of Earth Sciences and Resources, Chang’an University, Xi’an, Shaanxi 710061, China
2. Center of Urumqi Natural Resources Comprehensive Survey, China Geological Survey, Urumqi, Xinjiang 830000, China
3. Shihezi University, Shihezi, Xinjiang 832003, China

Received:2022-06-28 Revised:2023-03-19 Online:2023-06-10 Published:2023-07-20

摘要/Abstract

摘要：

为了研究玉米根际土壤细菌群落对土壤Cd污染的响应,本研究基于Illumina MiSeq高通量测序平台,对取自甘肃省白银市四龙镇地区的4组不同Cd污染程度的土壤样品进行16S rDNA扩增子测序分析。结果显示:4组不同Cd污染程度的玉米根际土壤细菌样品的Chao1指数和Shannon指数差异性不明显(p>0.05),表明Cd污染对土壤细菌多样性的影响不显著。4组样品的细菌丰富度相似,优势类群大致相同。4组样品的优势门均为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)和浮霉菌门(Planctomycetes),优势菌属为Ohtaekwangia、GP4、GP6、硝化螺旋菌属(Nitrospira)和芽单胞菌属(Gemmatimonas)等。Ohtaekwangia和芽单胞菌属的细菌抗逆性较强,随着环境中Cd含量的增加,其细菌相对丰度增加,而GP4和GP6的细菌抗逆性较差,其相对丰度会随土壤Cd含量的降低而降低。Cd污染会引起土壤中细菌的多样性变化,但细菌多样性与土壤中Cd含量并非简单的线性关系。土壤细菌多样性随着环境中Cd含量的增加,总体上呈先升高后降低的趋势,中等污染程度的土壤样品的细菌多样性指数最高。

关键词: 细菌多样性, Cd污染, 玉米根际土壤, 高通量测序

Abstract:

To explore the response of bacterial communities of corn in rhizospheric soil to Cd pollution, we utilized the Illumina MiSeq high throughput sequencing platform, and conducted 16S rDNA amplification analysis on four groups of soil samples with different levels of Cd pollution from Silong Town (Baiyin City, Gansu Pro-vince). The results showed no significant difference in the Chao1 and Shannon indices of soil bacterial samples from these four groups of Cd-polluted samples (p>0.05), indicating that the pollution effect is insignificant. The bacterial abundance of the four sample groups is similar, and the dominant communities are largely the same, i.e., (phylum level) Proteobacteria, Bacteroidetes, Acidobacteria, Chloroflexi and Planctomycetes, and (dominant genera) are Ohtaekwangia, GP4, GP6, Nitrospira and Gemmatimonas. The Ohtaekwangia and Gemmatimonas have high stress resistance, and their relative abundance increases with increasing soil Cd content. In contrast, GP4 and GP6 have low stress resistance, and their relative abundance decreases with decreasing soil Cd content. Cadmium pollution can cause changes in bacterial diversity in the soil, but there is no simple linear relationship between bacterial diversity and Cd content. With increasing Cd content in the environment, soil bacterial diversity generally increases first and then decreases. Soil samples with intermediate pollution level have the highest bacterial diversity.

Key words: bacterial diversity, Cd pollution, corn rhizosphere soil, high-throughput sequencing

中图分类号:

X142
X172

胡永浩, 段星星, 夏昭德, 韩宝华. 玉米根际土壤细菌群落对不同程度镉污染的响应: 以甘肃省白银市四龙镇地区为例[J]. 现代地质, 2023, 37(03): 758-766.

HU Yonghao, DUAN Xingxing, XIA Zhaode, HAN Baohua. Impacts of Cadmium Concentration in Rhizospheric Soil of Corn on Bacterial Communities: A Case Study from Silong Town, Baiyin City, Gansu Province[J]. Geoscience, 2023, 37(03): 758-766.

图/表 7

表1 甘肃省四龙镇地区土壤样品基本理化性质

Table 1 Basic properties of the soil samples in Silong Town, Gansu Province

编号	位置	样品数	pH	有机质(g/kg)	Cd(mg/kg)	Zn(mg/kg)	Pb(mg/kg)	Cu(mg/kg)
GJ1	民勤村东南和麻拉牌	4	8.71±0.10	0.61±0.21	24.63±18.14	107.0±16.8	34.9±6.5	41.2±1.3
GJ2	腾家窑北	11	8.62±0.05	0.83±0.12	1.97±0.29	130.9±21.1	40.0±5.8	37.1±6.8
GJ3	高崖村	5	8.32±0.04	0.84±0.35	0.32±0.01	81.7±25.9	24.2±5.0	25.0±0.9
GJ4	四龙村西	10	8.56±0.10	0.77±0.11	0.29±0.03	89.0±40.4	37.3±29.0	25.8±3.8

图1 甘肃省白银市四龙镇地区位置图(a)和土壤样品取样点分布图(b)

Fig.1 Location map of Silong Town, Baiyin City, Gansu Province (a) and distribution of sampling sites for soil samples (b)

图2 甘肃省四龙镇地区土壤细菌OTUs分布Venn图

Fig.2 Venn diagram for soil bacterial OTUs distribution in Silong Town, Gansu Province

图3 甘肃省四龙镇地区土壤细菌Chao1指数(a)与Shannon指数(b)箱体图

Fig.3 Box plot of soil bacterial Chao1 index (a) and Shannon index (b) in Silong Town, Gansu Province

图4 甘肃省四龙镇地区玉米根际土壤细菌门水平(a)和属水平(b)相对丰度柱状图

Fig.4 Histogram of relative soil bacterial abundance in corn rhizosphere at phyla level (a) and genus level (b) in Silong Town, Gansu Province

图5 甘肃省四龙镇地区不同镉污染程度下玉米根际土壤细菌属水平热图

Fig.5 Heat map of soil bacteria in corn rhizosphere (genus le-vel) under different Cd pollution levels in Silong Town, Gansu Province

图6 甘肃省四龙镇地区玉米根际土壤细菌主坐标(PCoA)分析图

Fig.6 Principal coordinate analysis (PCoA) of soil bacteria in corn rhizosphere in Silong Town, Gansu Province

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玉米根际土壤细菌群落对不同程度镉污染的响应: 以甘肃省白银市四龙镇地区为例

Impacts of Cadmium Concentration in Rhizospheric Soil of Corn on Bacterial Communities: A Case Study from Silong Town, Baiyin City, Gansu Province

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