The palynological analysis of 124 samples in this paper was carried out from B13, B26, B28, G6, G7, G10, G20, G27 and G39 wells of Dongying Formation, Nanpu sag, eastern Hebei Province. According to the sporopollen contents, one assemblage has been proposed which was characterized by the predominance of Ulmipollenites undulosus, with high content of Pinaceae among gymnospermous pollens and abundant Ulmipollenites, Quercoidites, Juglanspollenites and Betulaceae among angiospermous pollens. Four including the two caeval subassemblages can be recognized as Ed3, Ed2 and Ed1, namely, Ulmipollenites undulosusQuercoidites subassemblage, JuglanspollenitesTiliaepollenites subassemblage/PinaceaeChenopodipollis subassemblage and CedripitesPiceapollis subassemblage. Moreover, the detailed interpretation has been given on difference of the two coeval Ed2 subassemblages. Sporopollen assemblages from Dongying Formation in Nanpu sag were compared carefully with those from other sags in Bohai Bay Basin and the results show that Nanpu sag sporopollen assemblages possess similar features with others, but with some small differences. The studied assemblage with high content Ulmipollenites undulosus may be assigned to Oligocene in age based on distribution of major Gymnospermous pollens, Angiospermous pollens and the first occurrence of a few Neogene pollens.