关键词: 生物降解, 复合菌, GC-MS, 饱和烃, 芳烃

Abstract:

In this study, effective hydrocarbon degrading mixed bacteria (I strain) has been obtained by screening, and three kinds of oil (Xi 5-P10, Sanyuanhou, G1131-262) from three different wells respectively in Daqing oilfield have been degraded by experiments with different time intervals. Then GC-MS analysis has been carried out on the oils. The change of the distribution of the saturated hydrocarbons and aromatic hydrocarbons of the oil is probed into by quantitative analysis. The results show that strain I is a kind of high-efficient hydrocarbon degrading bacteria; the degradation abilities to the three kinds of crude oil by the same type of bacteria are different significantly, so the field test of hydrocarbon contamination by bioremediation requires different high-efficiency biodegrading bacteria depending on the property of the oil; that not only the past conclusions of the biodegradation orders of saturated hydrocarbons and aromatics biodegradation are verified, but also some new views are put forward. Such as the degradation of hopanoid is after the rearrangement steranes, naphthalene start to degrading earlier than phenanthrene and trimethylnaphthalene earlier than tri-methyl phenanthrene, tri-methylnaphthalene and tetra-methylnaphthalene achieve a balance after the depth of biodegradable and degradation rate slow down after that. When hydrocarbon is degraded to a certain stage,  PAHs of stronger anti-biodegradation is enriched which will increase the toxicity to the environment, so PAH-degrading bacteria or naphthalene, phenanthrene-degrading bacteria or other special effect bacteria will be a key of environment bioremedation.

Key words: biological degradation, mixed bacteria, GC-MS, saturated hydrocarbon, aromatic hydrocarbon