微生物修复石油污染地下水的实验研究

现代地质 ›› 2009, Vol. 23 ›› Issue (1): 120-124.

微生物修复石油污染地下水的实验研究

张胜¹，毕二平²，陈立¹ ，崔晓梅³ ,张翠云¹，韩占涛¹ ，赵红梅¹ ，张发旺¹* ，李政红¹

1中国地质科学院水文地质环境地质研究所，河北正定050803；
2中国地质大学水资源与环境学院，北京100083；3中国地质科学院，北京100037

收稿日期:2008-08-11 修回日期:2008-12-15 出版日期:2009-02-20 发布日期:2011-12-21
通讯作者: 张发旺,男,研究员, 1965年出生，水文地质、矿山环境地质专业，主要从事环境地质研究。
作者简介:张胜，男,副研究员, 1956年出生，微生物环境工程专业, 主要从事环境地质研究。 Email:zshenghb@sina.com。
基金资助:
科技部国际合作项目(2005DFA90200)

Experimental Study on Microbial Remediation of Petroleum Polluted Groundwater

ZHANG Sheng¹，BI Er-ping²，CHEN Li¹，CUI Xiao-mei³，ZHANG Cui-yun¹,HAN Zhan-tao¹ ，ZHAO Hong-mei¹，ZHANG Fa-wang ^1* ，LI Zheng-hong¹

1Institute of Hydrogeology and Environmental Geology, CAGS，Zhengding, Hebei050803，China;
2School of Water Resources and Environment, China University of Geosciences, Beijing100083，China; 
3Chinese Academy of Geological Sciences, Beijing100037，China

Received:2008-08-11 Revised:2008-12-15 Online:2009-02-20 Published:2011-12-21

摘要/Abstract

摘要：

为修复陕北黄土区石油污染地下水，采用优化土著微生物菌群的生物技术，进行了地下水中石油的降解与修复实验研究。在实验装置内加入了1.5%的优化菌群制剂，优化出的菌群初步鉴定主要有：假单胞菌属、微球菌属、放线菌属、真菌类的青霉属和曲霉属等。实验结果显示，在实验装置中人为添加石油含量为182.5 mg/l、862.5 mg/l、1 695.0 mg/l时，经过28~37 d的微生物修复，地下水中石油的降解率为27.47%~92.46%，而无菌对照中的石油含量变化在5%以内，说明在无菌条件下地下水中石油降解缓慢。该实验过程验证了微生物修复技术在地下水石油污染修复中的有效性，探索了应用的可行性。


关键词: 微生物修复, 石油污染, 地下水

Abstract:

In order to remediate oil contaminated groundwater in loess region of North Shaanxi Province, microbial techniques of optimized native microbial communities were used for experimental studies on oil polluted groundwater remediation. In this experiment, 1.5% optimized microbial populations were added. The optimized microbial populations were primarily identified as Pseudomonas, Micrococcus, Actinomayces, Penicillium and Aspergillus. The results of the experiment showed that the oil, with contents of 182.5 mg/l, 862.5 mg/l and 1,695.0 mg/l, added artificially into soil can be reduced by 27.47% to 92.46% after enhanced microbial remediation for 28 to 37 days, whereas the oil degradation rate in non-bacterium reference sample was less than 5%, which suggests that the oil degradation in groundwater with non-bacterium was slow. This experiment demonstrated the effectiveness of microbial remediation techniques for oil contaminated groundwater, and explored the feasibility of the technique application. 

Key words: microbial remediation, oil pollution, groundwater

中图分类号:

TE991.2

张胜毕二平陈立崔晓梅张翠云韩占涛赵红梅张发旺李政红. 微生物修复石油污染地下水的实验研究[J]. 现代地质, 2009, 23(1): 120-124.

ZHANG Sheng BI Er-ping CHEN Li CUI Xiao-mei ZHANG Cui-yun HAN Zhan-tao. Experimental Study on Microbial Remediation of Petroleum Polluted Groundwater[J]. Geoscience, 2009, 23(1): 120-124.