A Biodegradation Experiment of Crude Oils in Laboratory and Quantitative Evaluation

Abstract

Abstract:

In this study, effective hydrocarbon degrading mixed bacteria (I strain) has been obtained by screening, and three kinds of oil (Xi 5-P10, Sanyuanhou, G1131-262) from three different wells respectively in Daqing oilfield have been degraded by experiments with different time intervals. Then GC-MS analysis has been carried out on the oils. The change of the distribution of the saturated hydrocarbons and aromatic hydrocarbons of the oil is probed into by quantitative analysis. The results show that strain I is a kind of high-efficient hydrocarbon degrading bacteria; the degradation abilities to the three kinds of crude oil by the same type of bacteria are different significantly, so the field test of hydrocarbon contamination by bioremediation requires different high-efficiency biodegrading bacteria depending on the property of the oil; that not only the past conclusions of the biodegradation orders of saturated hydrocarbons and aromatics biodegradation are verified, but also some new views are put forward. Such as the degradation of hopanoid is after the rearrangement steranes, naphthalene start to degrading earlier than phenanthrene and trimethylnaphthalene earlier than tri-methyl phenanthrene, tri-methylnaphthalene and tetra-methylnaphthalene achieve a balance after the depth of biodegradable and degradation rate slow down after that. When hydrocarbon is degraded to a certain stage, PAHs of stronger anti-biodegradation is enriched which will increase the toxicity to the environment, so PAH-degrading bacteria or naphthalene, phenanthrene-degrading bacteria or other special effect bacteria will be a key of environment bioremedation.

Key words: biological degradation, mixed bacteria, GC-MS, saturated hydrocarbon, aromatic hydrocarbon

CLC Number:

TE122.1

XIANG Ting-Sheng, HEI Hua-Li. A Biodegradation Experiment of Crude Oils in Laboratory and Quantitative Evaluation[J]. Geoscience, 2010, 24(2): 259-267.

References

［1］Georgiana F da Cruz,Eugênio V dos Santos Neto,Anita J Marsaioli. Petroleum degradation by aerobic microbiota from the Pampo Sul Oil Field, Campos Basin, Brazil［J］. Organic Geochemistry, 2008(1): 204-209.
［2］张渠,宋晓莹,张志荣.原油中甾烷的生物降解模拟实验研究［J］. 石油实验地质，2007, 29(1)：99-102.
［3］Nadalig T, Raymond N,Gliewiczm, et al.Development of a protocol to study aerobic bacterial degradation of polycyclic aromatic hydrocarbons application to phenanthrenes［J］. Polycyclic Aromatic Compounds, 2000, 18(2): 177-191.
［4］Bost F D, FronteraSuau R, McDonald T J, et al. Aerobic biodegradation of hopanes and norhopanes in Venezuelan crude oils ［J］. Organic Geochemistry, 2001, 32(1): 105-114.
［5］Branimir Jovancˇic＇evic＇, Malisˇa Antic＇, Ivona Pavlovic＇. Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments［J］. Water Air Soil Pollut, 2008, 190(1): 299-307.
［6］L1via G S M Cordeiro,Renato S Carreira,Angela L R Wagener. Geochemistry of fecal sterols in a contaminated estuary in southeastern Brazil ［J］. Organic Geochemistry, 2008, 39(1): 97-103.
［7］包建平,朱俊章,朱翠山，等. 原油生物降解模拟实验［J］.石油勘探与开发, 2007, 35(1): 43-47.
［8］陈仲宇，刘明，朱忠云，等. 江汉盐湖盆地中高成熟原油的地球化学特征及油源探讨［J］.地球化学, 2009, 38(4): 379-386.
［9］黄海平,周树青,初振淼,等. 生物降解作用对原油中烷基菲分布的影响［J］. 现代地质，2005，19(3): 416-424.
［10］黄海平,杨杰,Larter S R. 生物降解作用对储层抽提物中多甲基取代萘分布的影响［J］. 地球科学, 2003, 28(3)：281-288.
［11］Greenwood P F. GCMS correlation of C3n series of naturally occurring highly branched alkanes and polypropylene oligomers ［J］. Organic Geochemistry, 2006, 37: 755-771.
［12］Annie Cazaussus, Pes R, Nicole Sellier,et al.GCMS and GCMSMS analysis of a complex essential oil［J］. Chromatographia, 1988, 25(10): 865-869.
［13］Daniel M White, Robert L Irvine. Potential applications for pyrolysis GC/MS in bioremediation ［J］. Environmental Monitoring and Assessment, 1998, 50(1)：53-65.
［14］Tissot B P, Welte D H. Petroleum Formation and Occurrence［M］. New York: SpringerVerlag, 1984:78-80.
［15］Peters K E, Moldowan J M. The Biomarker Guide: Interreting Molecular Fossils in Petroleum and Ancient Sediments ［M］. Englewood Cliffs, N J: Prentice Hall, 1993:50-60.
［16］Ben G K van Aarssen, Trevor P Bastow, Robert Alexander, et al. Distributions of methylated naphthalenes in crude oils:indicators of maturity, biodegradation and mixing［J］. Organic Geochemistry, 1999, 30: 1213-1227.